proprotein convertase 1 การใช้

• Proprotein convertase 1 is the enzyme largely responsible for the first step in the biosynthesis of insulin.
• Following the action of proprotein convertase 1, a carboxypeptidase is required to remove the basic residues from the processing intermediate and generate the bioactive form of insulin.
• There are nine subtilisin homologs in mammals; in addition to proprotein convertase 1 and 2, other members of this enzyme family include furin, SKI1 / S1P.
• The mice or humans, most likely due to the presence of the second convertase, although mice lacking proprotein convertase 1 activity show a number of defects including slow growth.
• Proprotein convertase 1 is a calcium ( Ca 2 + ) activated serine endoprotease ( meaning that a serine residue is part of the active site that hydrolyzes the peptide bond within the substrate ).
• Once released from the signal peptide, it undergoes additional proteolysis and posttranslational modification " ( indicated by "'^ "') " . 11 amino acids are removed from the N-terminus by the enzyme proprotein convertase 2 ( PC2 ) while 16 are removed from the C-terminus of the proIAPP molecule by proprotein convertase 1 / 3 ( PC1 / 3 ).